- Yukari Tanaka
- Team leader, Drug Metabolism & Pharmacokinetics, Research Laboratory for Development
- Masayuki Sugimoto
- Obesity & Metabolic Diseases, Drug Discovery & Disease Research Laboratory
MS is a useful approach to visualize the localization of endogenous molecules and administered compounds by using a mass spectrometer, without using any probes. It is hoped that this technology will be useful for elucidating the MoA for drug efficacy and toxicity, as well as for the analysis of drug distribution in tissues, and finding biomarkers by localized analysis of endogenous molecules, such as lipids, that are difficult to visualize with existing methods because of difficulties in the specific and quantitative analysis of target molecules.
A Shionogi group including Yukari Tanaka developed the quantitative MALDI-IMS method to resolve these issues, and was successful in analyzing drug distribution in tissues as well as the concentration in regions of interest within tissues. Using this method, Masayuki Sugimoto et al, in collaboration with scientists at Hokkaido University, were successful in visualizing the distributions of sphingomyelin molecular species in the brain sections of mice. Moreover they generated results that led to the identification of new diagnostic markers and analysis of lipid molecule physiological functions by studying the relationship with metabolic enzymes.
The establishment of high resolution imaging mass spectrometry (IMS) for absolute quantification of molecules and compounds in tissue sections
- 1. Nozomi Takai, Yukari Tanaka, and Hideo Saji
Quantification of small molecule drugs in biological tissue sections by imaging mass spectrometry using surrogate tissue-based calibration standards.
Mass Spectrom (Tokyo). 2014;3(1):A0025.
- 2. Nozomi Takai, Yukari Tanaka, Kazuhiro Inazawa, and Hideo Saji
Quantitative analysis of pharmaceutical drug distribution in multiple organs by imaging mass spectrometry.
Rapid Commun Mass Spectrom. 2012 Jul 15;26(13):1549-56.
- 3. Masayuki Sugimoto, Yoichi Shimizu, Takeshi Yoshioka, Masato Wakabayashi, Yukari Tanaka, Kenichi Higashino, Yoshito Numata, Shota Sakai, Akio Kihara, Yasuyuki Igarashi, Yuji Kuge.
Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains.
Biochim Biophys Acta. 2015 Dec;1851(12):1554-65.
- 4. Masayuki Sugimoto, Masato Wakabayashi, Yoichi Shimizu, Takeshi Yoshioka, Kenichi Higashino, Yoshito Numata, Tomohiko Okuda, Songji Zhao, Shota Sakai, Yasuyuki Igarashi, Yuji Kuge.
Imaging Mass Spectrometry Reveals Acyl-Chain- and Region-Specific Sphingolipid Metabolism in the Kidneys of Sphingomyelin Synthase 2-Deficient Mice.
PLoS One. 2016 Mar 24;11(3):e0152191.